4A99
STRUCTURE OF THE TETRACYCLINE DEGRADING MONOOXYGENASE TETX IN COMPLEX WITH MINOCYCLINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-28 |
| Detector | MARRESEARCH MAR165 |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.878, 80.334, 86.627 |
| Unit cell angles | 110.82, 90.27, 93.39 |
Refinement procedure
| Resolution | 80.940 - 2.180 |
| R-factor | 0.18713 |
| Rwork | 0.185 |
| R-free | 0.23476 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xdo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.918 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.940 | 2.310 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Rmerge | 0.100 | 0.620 |
| Number of reflections | 86443 | |
| <I/σ(I)> | 11.86 | 2.37 |
| Completeness [%] | 95.4 | 80.4 |
| Redundancy | 3.75 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
