4A8W
Non-Catalytic Ions Direct the RNA-Dependent RNA Polymerase of Bacterial dsRNA virus phi6 from De Novo Initiation to Elongation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.910, 91.430, 141.570 |
| Unit cell angles | 90.00, 101.92, 90.00 |
Refinement procedure
| Resolution | 44.510 - 3.040 |
| R-factor | 0.2112 |
| Rwork | 0.209 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hhs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Refinement software | BUSTER-TNT (BUSTER 2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | |
| High resolution limit [Å] | 3.000 | |
| Rmerge | 0.180 | |
| Number of reflections | 51249 | |
| <I/σ(I)> | 14.7 | 2.4 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12% PEG 20000, 100 MM MES (PH 6.5) |
