4A8M
Non-Catalytic Ions Direct the RNA-Dependent RNA Polymerase of Bacterial dsRNA virus phi6 from De Novo Initiation to Elongation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.270, 92.060, 140.810 |
| Unit cell angles | 90.00, 101.30, 90.00 |
Refinement procedure
| Resolution | 58.720 - 2.920 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.20910 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.150 |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.920 |
| Rmerge | 0.090 | |
| Number of reflections | 55288 | |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 95.0 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 4000, 100 MM HEPES (PH 7.5), 8.5% ISOPROPANOL, 15% GLYCEROL, 2 MM MNCL2 |
