4A75
The Lin28b Cold shock domain in complex with hexathymidine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY |
| Synchrotron site | BESSY |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.799, 81.822, 99.819 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.910 - 1.750 |
| R-factor | 0.17239 |
| Rwork | 0.171 |
| R-free | 0.19801 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.476 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.910 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | |
| Rmeas | 0.078 | 0.390 |
| Number of reflections | 39188 | |
| <I/σ(I)> | 13.2 | 3.3 |
| Completeness [%] | 89.4 | 87.8 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.75 mM protein-DNA complex (in 20 mM Tris/HCl, pH 7.5, 100 mM KCl, 2 mM MgCl2 and 1 mM DTT) and 0.1 mM Bis-Tris, pH 5.5, 15% (w/v) PEG 3350 and 0.1 M sodium thiocyanate as reservoir buffer |
