4A6Y
CRYSTAL STRUCTURE OF FAB FRAGMENT OF ANTI-(4-HYDROXY-3-NITROPHENYL) -ACETYL MURINE GERMLINE ANTIBODY BBE6.12H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.283, 81.418, 128.911 |
| Unit cell angles | 90.00, 90.50, 90.00 |
Refinement procedure
| Resolution | 23.020 - 2.900 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ngq |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.700 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.120 | 0.420 |
| Number of reflections | 19688 | |
| <I/σ(I)> | 4.7 | 2.6 |
| Completeness [%] | 93.5 | 87.3 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 21% PEG 3350 IN 0.05 M TRIS, PH 7.1 |
