4A48

Crosstalk between Cu(I) and Zn(II) homeostasis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-30
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	24.660, 52.600, 39.920
Unit cell angles	90.00, 98.13, 90.00

Refinement procedure

Resolution	39.520 - 1.400
R-factor	0.14263
Rwork	0.140
R-free	0.19527
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xmw
RMSD bond length	0.011
RMSD bond angle	1.525
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.590	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.050	0.220
Number of reflections	18146
<I/σ(I)>	15.3	5.1
Completeness [%]	91.4	86.2
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				200 MM NAF, 20% (W/V) PEG 3350