4A47
Crosstalk between Cu(I) and Zn(II) homeostasis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-30 |
| Detector | ADSC CCD |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 85.620, 85.620, 188.142 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.980 - 1.900 |
| R-factor | 0.19706 |
| Rwork | 0.195 |
| R-free | 0.24302 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.471 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.810 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.370 |
| Number of reflections | 21226 | |
| <I/σ(I)> | 10.9 | 4.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.6 M TRISODIUM CITRATE, PH 6.5. |
