4A46
Crosstalk between Cu(I) and Zn(II) homeostasis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.710, 41.990, 45.240 |
| Unit cell angles | 83.34, 89.52, 75.28 |
Refinement procedure
| Resolution | 44.920 - 1.850 |
| R-factor | 0.19416 |
| Rwork | 0.192 |
| R-free | 0.24279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmt |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.488 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.920 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 0.200 |
| Number of reflections | 19967 | |
| <I/σ(I)> | 5.1 | 2.1 |
| Completeness [%] | 91.5 | 90.9 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 200 MM MGCL2 100 MM TRIS PH 8.5 30% PEG 4000 |
