4A3R
Crystal structure of Enolase from Bacillus subtilis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 4 |
| Unit cell lengths | 187.074, 187.074, 56.501 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.830 - 2.200 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.21770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iyx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.072 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.520 |
| Number of reflections | 96029 | |
| <I/σ(I)> | 9.9 | 1.9 |
| Completeness [%] | 96.2 | 90.8 |
| Redundancy | 2.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.0 M TRI-SODIUM CITRATE 0.1 M, NA CACODYLATE PH 6.5 . |
