Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200H |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-10-17 |
Detector | RIGAKU-MSC |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.385, 86.127, 152.637 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.630 - 1.800 |
R-factor | 0.15532 |
Rwork | 0.154 |
R-free | 0.18722 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v10 |
RMSD bond length | 0.032 |
RMSD bond angle | 2.238 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.630 | 1.847 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.050 | 0.300 |
Number of reflections | 63965 | |
<I/σ(I)> | 9.5 | 2.2 |
Completeness [%] | 96.6 | 90.34 |
Redundancy | 3.2 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | PEG 1000, PEG8000., pH 5.5 |