4A28
Eg5-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-11-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.900, 78.390, 92.690 |
| Unit cell angles | 90.00, 93.16, 90.00 |
Refinement procedure
| Resolution | 29.505 - 2.550 |
| R-factor | 0.1842 |
| Rwork | 0.180 |
| R-free | 0.26370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ii6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.472 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.090 | 0.290 |
| Number of reflections | 12271 | |
| <I/σ(I)> | 10.7 | 5.5 |
| Completeness [%] | 98.8 | 97.9 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | pH 5.5 |
