4A22
Structure of Mycobacterium tuberculosis fructose 1,6-bisphosphate aldolase bound to N-(4-hydroxybutyl)- glycolohydroxamic acid bis- phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 335.386, 42.977, 102.601 |
| Unit cell angles | 90.00, 99.39, 90.00 |
Refinement procedure
| Resolution | 45.415 - 1.900 |
| R-factor | 0.1825 |
| Rwork | 0.179 |
| R-free | 0.22060 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.188 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.900 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.080 |
| Number of reflections | 114281 |
| <I/σ(I)> | 10 |
| Completeness [%] | 99.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 8000, 1.8 M LI2SO4, 50 MM TRIS/HOAC PH 7.5. |
