4A11
Structure of the hsDDB1-hsCSA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 138.330, 138.330, 244.970 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.067 - 3.310 |
| R-factor | 0.1785 |
| Rwork | 0.176 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ei3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.342 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.390 |
| High resolution limit [Å] | 3.300 | 3.310 |
| Rmerge | 0.100 | 0.430 |
| Number of reflections | 38124 | |
| <I/σ(I)> | 7.03 | 1.95 |
| Completeness [%] | 92.4 | 78.4 |
| Redundancy | 2.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 1.4-1.58 M NAKPO4, 0.1 M NAMALONATE, 0-0.1 M LI2SO4, pH 8.0 |
