4A10
Apo-structure of 2-octenoyl-CoA carboxylase reductase CinF from streptomyces sp.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-09-08 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 101.640, 85.220, 113.970 |
Unit cell angles | 90.00, 107.45, 90.00 |
Refinement procedure
Resolution | 48.480 - 2.250 |
R-factor | 0.2124 |
Rwork | 0.209 |
R-free | 0.26867 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3krt |
RMSD bond length | 0.010 |
RMSD bond angle | 1.333 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.480 | 2.250 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.160 | 0.620 |
Number of reflections | 107076 | |
<I/σ(I)> | 8.78 | 3.7 |
Completeness [%] | 98.6 | 98.3 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.1M TRIS PH7, 20% GLYCEROL, 12% PEG8000 |