4A0N
Crystal structure of Survivin bound to the phosphorylated N-terminal tail of histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 68.455, 68.234, 89.965 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.366 - 2.743 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qfa |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.774 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.500 | 2.900 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.070 | 0.630 |
| Number of reflections | 5652 | |
| <I/σ(I)> | 14.8 | 2.2 |
| Completeness [%] | 2.7 | 87.7 |
| Redundancy | 5.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5, 0.2 M LISO4, 40% PEG 400. |
