4A0J
Crystal structure of Survivin bound to the phosphorylated N-terminal tail of histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.365, 70.896, 81.465 |
| Unit cell angles | 90.00, 129.03, 90.00 |
Refinement procedure
| Resolution | 56.937 - 2.803 |
| R-factor | 0.2011 |
| Rwork | 0.198 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qfa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.024 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.200 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | 0.620 |
| Number of reflections | 12491 | |
| <I/σ(I)> | 12.1 | 2.1 |
| Completeness [%] | 99.4 | 96.1 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 0.1 M MES PH 6.0, 18% PEG 4000. |
