4A08
Structure of hsDDB1-drDDB2 bound to a 13 bp CPD-duplex (purine at D-1 position) at 3.0 A resolution (CPD 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 114.370, 116.700, 137.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.620 - 3.000 |
| R-factor | 0.23703 |
| Rwork | 0.234 |
| R-free | 0.29418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ei1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.085 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 | 0.590 |
| Number of reflections | 37332 | |
| <I/σ(I)> | 14.2 | 4.1 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 100 MM MES, 25 MM NAOH, 18% PEG 350MME., pH 5.6 |
