4A02
X-ray crystallographic structure of EfCBM33A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-08 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 32 |
| Unit cell lengths | 52.109, 52.109, 59.653 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.900 - 0.950 |
| R-factor | 0.0964 |
| R-free | 0.11580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bem |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.032 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.900 | 1.000 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Number of reflections | 112000 | |
| <I/σ(I)> | 12.1 | 3.4 |
| Completeness [%] | 96.0 | 86.6 |
| Redundancy | 2.6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 1.0 M K/NA TARTRATE, 0.1 M IMIDAZOLE PH 8.0 AND 0.2 M NACL. |
