488D
CATALYTIC RNA ENZYME-PRODUCT COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-15 |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.503, 65.503, 136.484 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.600 - 3.100 |
| R-factor | 0.258 |
| Rwork | 0.258 |
| R-free | 0.30100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 35.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.600 | 3.400 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.275 | |
| Total number of observations | 10825 * | |
| Number of reflections | 6553 | 834 * |
| Completeness [%] | 95.5 | 91.3 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 | 16 * | pH 5.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ribozyme | 1 (mM) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | 1.8 (M) | ||
| 4 | 1 | reservoir | 50 (mM) | ||
| 5 | 1 | reservoir | 1.8 (M) | ||
| 6 | 1 | reservoir | EDTA | 1.0 (mM) |
