486D
X-RAY CRYSTAL STRUCTURES OF 70S RIBOSOME FUNCTIONAL COMPLEXES
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 105 |
Detector technology | AREA DETECTOR |
Wavelength(s) | 1.1051, 1.1055, 1.0764, 1.1047 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 508.020, 508.020, 802.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | |
High resolution limit [Å] | 7.500 |
Rmerge | 0.089 |
Number of reflections | 124437 |
<I/σ(I)> | 15.8 |
Completeness [%] | 97.7 |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging and sitting drop * | 7.4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 20 (mM) | ||
2 | 1 | drop | 100 (mM) | ||
3 | 1 | drop | Tris-HCl | 20 (mM) |