486D

X-RAY CRYSTAL STRUCTURES OF 70S RIBOSOME FUNCTIONAL COMPLEXES

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	105
Detector technology	AREA DETECTOR
Wavelength(s)	1.1051, 1.1055, 1.0764, 1.1047
Spacegroup name	I 4 2 2
Unit cell lengths	508.020, 508.020, 802.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 7.500
Structure solution method	MAD PHASING
Starting model (for MR)	4tna 3tra 1pbr 1gid 430d
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE

Data quality characteristics

	Overall
High resolution limit [Å]	7.500
Rmerge	0.089
Number of reflections	124437
<I/σ(I)>	15.8
Completeness [%]	97.7
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging and sitting drop *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		20 (mM)
2	1	drop		100 (mM)
3	1	drop	Tris-HCl	20 (mM)