479D
CRYSTAL STRUCTURE A DNA:RNA HYBRID DUPLEX, A DYNAMIC MODEL FOR RNASE H RECOGNITION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 263 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 61 |
| Unit cell lengths | 49.150, 49.150, 46.130 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.179 * |
| Rwork | 0.199 |
| R-free | 0.20500 * |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.260 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.380 |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.067 * |
| Number of reflections | 5382 * |
| Completeness [%] | 91.0 * |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | DROP: 1 MM HYBID (DOUBLE STRAND), 400 MM MGCL2, 0.10 M SODIUM CACODYLATE (PH 6.0), 2MM SPERMINE TETRACHLORIDE, 5% MPD; RESERVOIR: 40 % MPD, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MGCL2 | ||
| 2 | 1 | 1 | SODIUM CACODYLATE | ||
| 3 | 1 | 1 | SPERMINE TETRACHLORIDE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mM) | |
| 2 | 1 | drop | 400 (mM) | ||
| 3 | 1 | drop | spermine tetrachloride | 2 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 100 (mM) | |
| 5 | 1 | drop | 5 (%(v/v)) | ||
| 6 | 1 | reservoir | MPD | 40 (%) |
