479D
CRYSTAL STRUCTURE A DNA:RNA HYBRID DUPLEX, A DYNAMIC MODEL FOR RNASE H RECOGNITION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 263 |
Detector technology | IMAGE PLATE |
Collection date | 1998-12-15 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 61 |
Unit cell lengths | 49.150, 49.150, 46.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.900 |
R-factor | 0.179 * |
Rwork | 0.199 |
R-free | 0.20500 * |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.260 * |
Data reduction software | DENZO |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 31.380 |
High resolution limit [Å] | 1.800 * |
Rmerge | 0.067 * |
Number of reflections | 5382 * |
Completeness [%] | 91.0 * |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | DROP: 1 MM HYBID (DOUBLE STRAND), 400 MM MGCL2, 0.10 M SODIUM CACODYLATE (PH 6.0), 2MM SPERMINE TETRACHLORIDE, 5% MPD; RESERVOIR: 40 % MPD, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MGCL2 | ||
2 | 1 | 1 | SODIUM CACODYLATE | ||
3 | 1 | 1 | SPERMINE TETRACHLORIDE | ||
4 | 1 | 1 | MPD | ||
5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1 (mM) | |
2 | 1 | drop | 400 (mM) | ||
3 | 1 | drop | spermine tetrachloride | 2 (mM) | |
4 | 1 | drop | sodium cacodylate | 100 (mM) | |
5 | 1 | drop | 5 (%(v/v)) | ||
6 | 1 | reservoir | MPD | 40 (%) |