474D
A NOVEL END-TO-END BINDING OF TWO NETROPSINS TO THE DNA DECAMER D(CCCCCIIIII)2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU |
Spacegroup name | P 1 |
Unit cell lengths | 32.660, 32.770, 37.710 |
Unit cell angles | 86.01, 84.37, 68.90 |
Refinement procedure
Resolution | 8.000 - 2.400 |
R-factor | 0.199 * |
Rwork | 0.190 |
R-free | 0.29200 * |
RMSD bond length | 0.015 * |
RMSD bond angle | 2.700 * |
Data reduction software | MSC |
Data scaling software | MSC |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.061 * |
Number of reflections | 4284 * |
Completeness [%] | 73.0 |
Redundancy | 2.04 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 291.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
2 | 1 | 1 | NETROPSIN | ||
3 | 1 | 1 | NA CACODYLATE | ||
4 | 1 | 1 | [CO(NH3)6]CL3 | ||
5 | 1 | 2 | WATER | ||
6 | 1 | 2 | NETROPSIN | ||
7 | 1 | 2 | NA CACODYLATE | ||
8 | 1 | 2 | [CO(NH3)6]CL3 | ||
9 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | oligonucleotide | 0.3 (mM) | |
2 | 1 | drop | netropsin | 0.3 (mM) | |
3 | 1 | drop | sodium cacodylate | 0.4 (mM) | |
4 | 1 | drop | cobaltic hexamine chloride | 0.06 (mM) | |
5 | 1 | reservoir | MPD | 60 (%) |