469D
CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 1998-02-07 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 41.200, 34.440, 46.620 |
| Unit cell angles | 90.00, 92.40, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25800 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.890 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.055 |
| Total number of observations | 39271 * |
| Number of reflections | 7032 |
| Completeness [%] | 99.4 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 295 | DROPLETS CONTAINING 2MM OLIGONUCLEOTIDE, 50 MM MGCL2, 50 MM NA HEPES PH 6.2, 15% PEG 400, AGAINST 1 ML OF RESERVOIR SOLUTION CONTAINING 100 MM MGCL2, 100 MM NA HEPES PH 6.2, 30% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MGCL2 | ||
| 2 | 1 | 1 | NA HEPES | ||
| 3 | 1 | 1 | PEG 400 | ||
| 4 | 1 | 2 | MGCL2 | ||
| 5 | 1 | 2 | NA HEPES | ||
| 6 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 4 (mM) | |
| 2 | 1 | reservoir | Na-HEPES | 50 (mM) | pH6.2 |
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | PEG400 | 15 (%(v/v)) |
