466D
DISORDER AND TWIN REFINEMENT OF RNA HEPTAMER DOUBLE HELIX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02-03 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 32.900, 47.530, 26.200 |
| Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.160 |
| R-factor | 0.15 * |
| Rwork | 0.147 |
| R-free | 0.24000 * |
| Structure solution method | SIRAS |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.017 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.170 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.069 | 0.564 |
| Total number of observations | 66567 * | |
| Number of reflections | 13258 * | |
| Completeness [%] | 96.8 | 93.3 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | refer to Mueller, U., (1999) RNA, 5, 670. * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SPERMINE | ||
| 2 | 1 | 1 | SODIUM CACODYLATE | ||
| 3 | 1 | 1 | SRCL2 | ||
| 4 | 1 | 1 | LICL | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 2 | MPD |
