466D
DISORDER AND TWIN REFINEMENT OF RNA HEPTAMER DOUBLE HELIX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-02-03 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 32.900, 47.530, 26.200 |
Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.160 |
R-factor | 0.15 * |
Rwork | 0.147 |
R-free | 0.24000 * |
Structure solution method | SIRAS |
RMSD bond length | 0.022 |
RMSD bond angle | 0.017 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.170 |
High resolution limit [Å] | 1.160 | 1.160 |
Rmerge | 0.069 | 0.564 |
Total number of observations | 66567 * | |
Number of reflections | 13258 * | |
Completeness [%] | 96.8 | 93.3 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | refer to Mueller, U., (1999) RNA, 5, 670. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SPERMINE | ||
2 | 1 | 1 | SODIUM CACODYLATE | ||
3 | 1 | 1 | SRCL2 | ||
4 | 1 | 1 | LICL | ||
5 | 1 | 1 | MPD | ||
6 | 1 | 2 | MPD |