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466D

DISORDER AND TWIN REFINEMENT OF RNA HEPTAMER DOUBLE HELIX

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsEMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron siteEMBL/DESY, HAMBURG
BeamlineX11
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date1997-02-03
DetectorMARRESEARCH
Spacegroup nameC 1 2 1
Unit cell lengths32.900, 47.530, 26.200
Unit cell angles90.00, 101.90, 90.00
Refinement procedure
Resolution15.000 - 1.160
R-factor0.15

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Rwork0.147
R-free0.24000

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Structure solution methodSIRAS
RMSD bond length0.022
RMSD bond angle0.017
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Refinement softwareSHELXL-97
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.0001.170
High resolution limit [Å]1.1601.160
Rmerge0.0690.564
Total number of observations66567

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Number of reflections13258

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Completeness [%]96.893.3
Redundancy4.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6293refer to Mueller, U., (1999) RNA, 5, 670.

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Crystallization Reagents
IDcrystal IDsolution IDreagent nameconcentrationdetails
111SPERMINE
211SODIUM CACODYLATE
311SRCL2
411LICL
511MPD
612MPD

227111

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