45TX

FAST-DM complex

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0001
Spacegroup name	P 41 21 2
Unit cell lengths	45.190, 45.190, 105.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.260 - 1.660
R-factor	0.2087
Rwork	0.207
R-free	0.23210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.929
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (v 5.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.520	1.660
High resolution limit [Å]	1.630	1.630
Rmeas	0.129	2.760
Number of reflections	14226	689
<I/σ(I)>	12.5
Completeness [%]	99.5
Redundancy	22.4
CC(1/2)	0.999	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M magnesium formate pH 5.3-5.6 and PEG 3350 16-26%, 1mM DM ligand