45SD

FAST_apo_crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0001
Spacegroup name	P 41 21 2
Unit cell lengths	45.510, 45.510, 105.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.790 - 2.900
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (v 5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.790	3.000
High resolution limit [Å]	2.900	2.900
Rmeas	0.073	0.691
Number of reflections	2936	413
<I/σ(I)>	26.2
Completeness [%]	99.9
Redundancy	9.7
CC(1/2)	0.999	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293.15	0.2 M magnesium formate pH 5.3-5.6 and PEG 3350 16-26%