44MS
VcFadRqm, a quadruple mutant (Y153A, K156E, L203F, L208F) of fatty acid metabolism regulator protein from Vibrio cholerae, in complex with oleoyl-CoA
Replaces: 8Y41Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-08-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.726, 57.335, 57.483 |
| Unit cell angles | 67.92, 63.35, 90.02 |
Refinement procedure
| Resolution | 19.490 - 2.580 |
| R-factor | 0.2577 |
| Rwork | 0.257 |
| R-free | 0.27610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.829 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.0_5936) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.780 |
| High resolution limit [Å] | 2.579 | 2.579 |
| Rmerge | 0.069 | |
| Number of reflections | 16083 | 15530 |
| <I/σ(I)> | 20.699 | |
| Completeness [%] | 96.7 | |
| Redundancy | 3 | |
| CC(1/2) | 0.020 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 300 | 0.05M Cadmium sulfate hydrate, 0.1M Hepes,1M sodium acetate trihydrate |
