44MO

VcFadRqm, a quadruple mutant (Y153A, K156E, L203F, L208F) of fatty acid metabolism regulator protein from Vibrio cholerae, in complex with DNA

Replaces:  8Y3Z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9778
Spacegroup name	P 43 21 2
Unit cell lengths	99.303, 99.303, 225.826
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.810 - 2.500
R-factor	0.1993
Rwork	0.198
R-free	0.21730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.000
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	PHENIX (2.0_5936)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.740
High resolution limit [Å]	2.500	2.500
Rmerge	0.012
Number of reflections	39940	35669
<I/σ(I)>	21.3
Completeness [%]	99.9	99.9
Redundancy	2.52
CC(1/2)		0.010

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL	7.5	289.15	16%(w/v)PEG8000,40mM KH2PO4,20%(v/v) Glycerol