44LS
Crystal structure of the fusion protein of T-lineage protein Themis and adaptor protein Grb2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 82 |
| Detector technology | CCD |
| Collection date | 2023-03-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979176 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.804, 136.544, 116.523 |
| Unit cell angles | 90.00, 112.61, 90.00 |
Refinement procedure
| Resolution | 35.940 - 3.060 |
| R-factor | 0.2609 |
| Rwork | 0.260 |
| R-free | 0.28600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.417 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.940 | 3.170 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Number of reflections | 27234 | 27234 |
| <I/σ(I)> | 9.16 | |
| Completeness [%] | 97.7 | |
| Redundancy | 5.8 | |
| CC(1/2) | 0.997 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1 M Tris-HCI pH 8.5, 2% (v/v) Tacsimate pH 8.0, 16% (w/v) polyethylene glycol 3350 |
