446D
STRUCTURE OF THE OLIGONUCLEOTIDE D(CGTATATACG) AS A SITE SPECIFIC COMPLEX WITH NICKEL IONS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 52.460, 52.460, 101.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.064 * | 0.405 |
| Total number of observations | 59339 * | |
| Number of reflections | 3015 * | |
| Completeness [%] | 94.2 * | 69.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA duplex | 0.2 (mM) | |
| 2 | 1 | drop | MPD | 4 (%) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | reservoir | MPD | 8 (%) | |
| 5 | 1 | reservoir | Na cacodylate | 25 (mM) |
