439D
5'-R(*CP*UP*GP*GP*GP*CP*GP*G)-3', 5'-R(*CP*CP*GP*CP*CP*UP*GP*G)-3'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-04 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 41.969, 41.969, 127.137 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.600 |
| R-factor | 0.183 * |
| Rwork | 0.224 |
| R-free | 0.24100 * |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.066 | 0.291 * |
| Total number of observations | 43171 * | |
| Number of reflections | 5994 | |
| Completeness [%] | 96.1 | 99.7 |
| Redundancy | 4.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 0.25 (mM) | |
| 2 | 1 | drop | spermine tetrahydrochloride | 12 (mM) | |
| 3 | 1 | drop | 80 (mM) | ||
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 6 | 1 | drop | MPD | 10 (%(v/v)) | |
| 7 | 1 | reservoir | MPD | 40 (%) |
