439D

5'-R(*CP*UP*GP*GP*GP*CP*GP*G)-3', 5'-R(*CP*CP*GP*CP*CP*UP*GP*G)-3'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	170
Detector technology	IMAGE PLATE
Collection date	1998-04-04
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	41.969, 41.969, 127.137
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.600
R-factor	0.183 *
Rwork	0.224
R-free	0.24100 *
RMSD bond length	0.023
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.066	0.291 *
Total number of observations	43171 *
Number of reflections	5994
Completeness [%]	96.1	99.7
Redundancy	4.3	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNA	0.25 (mM)
2	1	drop	spermine tetrahydrochloride	12 (mM)
3	1	drop		80 (mM)
4	1	drop		20 (mM)
5	1	drop	sodium cacodylate	40 (mM)
6	1	drop	MPD	10 (%(v/v))
7	1	reservoir	MPD	40 (%)