438D

STRUCTURE OF AN RNA DUPLEX R(GGGCGCUCC)2 WITH NON-ADJACENT G.U BASE PAIRS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	H 3
Unit cell lengths	45.680, 45.680, 59.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.196 *
Rwork	0.186
R-free	0.24000
RMSD bond length	0.010 *
RMSD bond angle	1.400 *
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.500
Rmerge	0.043
Number of reflections	1403
Completeness [%]	83.8
Redundancy	37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNA	1 (mM)	single strand
2	1	drop	sodium cacodylate	25 (mM)
3	1	drop		1 (mM)
4	1	drop	spermine tetrachloride	0.5 (mM)
5	1	reservoir	MPD	40 (%)