438D
STRUCTURE OF AN RNA DUPLEX R(GGGCGCUCC)2 WITH NON-ADJACENT G.U BASE PAIRS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | H 3 |
Unit cell lengths | 45.680, 45.680, 59.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.196 * |
Rwork | 0.186 |
R-free | 0.24000 |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.400 * |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.043 |
Number of reflections | 1403 |
Completeness [%] | 83.8 |
Redundancy | 37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | RNA | 1 (mM) | single strand |
2 | 1 | drop | sodium cacodylate | 25 (mM) | |
3 | 1 | drop | 1 (mM) | ||
4 | 1 | drop | spermine tetrachloride | 0.5 (mM) | |
5 | 1 | reservoir | MPD | 40 (%) |