431D
5'-D(*GP*GP*CP*CP*AP*AP*TP*TP*GP*G)-3'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.106, 36.455, 52.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.150 |
| R-factor | 0.167 * |
| Starting model (for MR) | UDJ049 USED AS STARTING MODEL FOR REFINEMENT |
| RMSD bond length | 0.017 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | SHELXL-93 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.180 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.054 | 0.158 |
| Total number of observations | 63114 * | |
| Number of reflections | 17760 | |
| <I/σ(I)> | 25 | 11.71 |
| Completeness [%] | 96.7 | 95 * |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6 | 289. * | pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | oligonucleotide | 0.41 (mM) | ||
| 2 | 1 | buffer | 10.6 (mM) | ||
| 5 | 1 | 1 | spermine | 0.22 (mM) | |
| 6 | 1 | 2 | MPD | 40 (%) |
