427D
5'-D(*CP*GP*CP*(CH2-DM1)GP*CP*G)-3'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-06-15 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 27.818, 27.818, 51.858 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.100 |
R-factor | 0.101 * |
Starting model (for MR) | DDF023 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.120 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.041 | 0.094 |
Number of reflections | 8581 | 338 * |
<I/σ(I)> | 25.27 | 10.58 |
Completeness [%] | 97.3 * | 77 |
Redundancy | 4.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 16 * | pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SODIUM CACODYLATE | ||
2 | 1 | 1 | NACL | ||
3 | 1 | 1 | BACL2 | ||
4 | 1 | 1 | SPERMINE | ||
5 | 1 | 1 | MPD | ||
6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | oligonucleotide | 4.0 (mM) | |
2 | 1 | drop | daunomycin HCl | 6.4 (mM) | |
3 | 1 | drop | MPD | 10 (%) | |
4 | 1 | drop | spermine tetrachloride | 12 (mM) | |
5 | 1 | drop | 80 (mM) | ||
6 | 1 | drop | 20 (mM) | ||
7 | 1 | drop | sodium cacodylate | ||
8 | 1 | reservoir | MPD | 35 (%) |