427D
5'-D(*CP*GP*CP*(CH2-DM1)GP*CP*G)-3'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 27.818, 27.818, 51.858 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.100 |
| R-factor | 0.101 * |
| Starting model (for MR) | DDF023 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.120 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.041 | 0.094 |
| Number of reflections | 8581 | 338 * |
| <I/σ(I)> | 25.27 | 10.58 |
| Completeness [%] | 97.3 * | 77 |
| Redundancy | 4.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 16 * | pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | BACL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 4.0 (mM) | |
| 2 | 1 | drop | daunomycin HCl | 6.4 (mM) | |
| 3 | 1 | drop | MPD | 10 (%) | |
| 4 | 1 | drop | spermine tetrachloride | 12 (mM) | |
| 5 | 1 | drop | 80 (mM) | ||
| 6 | 1 | drop | 20 (mM) | ||
| 7 | 1 | drop | sodium cacodylate | ||
| 8 | 1 | reservoir | MPD | 35 (%) |
