426D
THE STRUCTURE OF MOST STUDIED DNA FRAGMENT CHANGES UNDER THE INFLUENCE OF IONS: A NEW PACKING OF D(CGCGAATTCGCG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-21 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | H 3 |
Unit cell lengths | 41.960, 41.960, 101.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 3.000 |
R-factor | 0.193 |
Rwork | 0.193 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 18.000 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.110 |
Number of reflections | 1262 |
Completeness [%] | 95.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PROTAMINE | ||
2 | 1 | 1 | CACL2 | ||
3 | 1 | 1 | MPD | ||
4 | 1 | 1 | CACODYLIC ACID | ||
5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | dodecamer | ||
2 | 1 | drop | 0.2 (M) | ||
3 | 1 | drop | protamine | ||
4 | 1 | drop | MPD | ||
5 | 1 | drop | cacodylic acid | ||
6 | 1 | reservoir | MPD |