425D
5'-D(*AP*CP*CP*GP*GP*TP*AP*CP*CP*GP*GP*T)-3'
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 120 |
Detector technology | AREA DETECTOR |
Collection date | 1995-03-10 |
Detector | XENTRONICS |
Spacegroup name | P 43 |
Unit cell lengths | 40.207, 40.207, 57.575 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.500 - 2.800 |
R-factor | 0.219 |
Rwork | 0.219 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IDEAL B-DNA |
RMSD bond length | 0.010 |
RMSD bond angle | 16.900 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | ULTIMA |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.500 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.056 | 0.210 |
Total number of observations | 13447 * | |
Number of reflections | 2243 | 2164 * |
Completeness [%] | 97.5 | 86.8 * |
Redundancy | 6 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 4 * | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MGCL2 | ||
2 | 1 | 1 | MNCL2 | ||
3 | 1 | 1 | SPERMINE | ||
4 | 1 | 1 | SODIUM CACODYLATE | ||
5 | 1 | 1 | MPD | ||
6 | 1 | 2 | SODIUM CACODYLATE | ||
7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA | 2 (mg/ml) | |
2 | 1 | drop | 32 (mM) | ||
3 | 1 | drop | 3-10 (mM) | ||
4 | 1 | drop | spermine tetrachloride | 7 (mM) | |
5 | 1 | drop | sodium cacodylate | 20 (mM) | |
6 | 1 | drop | MPD | 5 (%(w/v)) | |
7 | 1 | reservoir | 18 (%) | ||
8 | 1 | reservoir | sodium cacodylate | 100 (mM) |