422D
5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.340, 39.980, 32.470 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| Rwork | 0.188 |
| R-free | 0.22800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | OCTAMER SEGMENT FROM 14-MER AUAUAUAUAUAUAU |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.045 | |
| Number of reflections | 2494 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 91.0 | 71 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 293 | pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 0.2 (M) | ||
| 2 | 1 | drop | magnesium acetate | 0.1 (M) | |
| 3 | 1 | drop | spermine | 0.6 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 0.05 (M) | pH6.5 |
| 5 | 1 | drop | PEG8000 | 10 (%) | |
| 6 | 1 | reservoir | PEG400 | 20 (%) |
