422D

5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	290
Detector technology	IMAGE PLATE
Spacegroup name	C 1 2 1
Unit cell lengths	71.340, 39.980, 32.470
Unit cell angles	90.00, 104.70, 90.00

Refinement procedure

Resolution	10.000 - 2.600
Rwork	0.188
R-free	0.22800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	OCTAMER SEGMENT FROM 14-MER AUAUAUAUAUAUAU
RMSD bond length	0.010
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.760
High resolution limit [Å]	2.600	2.600
Rmerge	0.045
Number of reflections	2494
<I/σ(I)>		2.1
Completeness [%]	91.0	71
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	293	pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		0.2 (M)
2	1	drop	magnesium acetate	0.1 (M)
3	1	drop	spermine	0.6 (mM)
4	1	drop	sodium cacodylate	0.05 (M)	pH6.5
5	1	drop	PEG8000	10 (%)
6	1	reservoir	PEG400	20 (%)