419D

OCTAMER 5'-R(*GP*UP*AP*UP*AP*CP*A)-D(P*C)-3' WITH SIX WATSON-CRICK BASE-PAIRS AND TWO 3' OVERHANG RESIDUES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	295
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	24.190, 45.530, 74.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.183 *
Rwork	0.186
R-free	0.25600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	R(GUGCACA)DC
RMSD bond length	0.008 *
RMSD bond angle	1.190 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.200
Rmerge	0.037
Number of reflections	3592 *
Completeness [%]	83.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNA	1 (mM)	single stranded conc.
2	1	drop	sodium cacodylate	50 (mM)
3	1	drop		10 (mM)
4	1	drop	spermine tetrachloride	25 (mM)
5	1	drop	MPD	2.4 (%(v/v))
6	1	reservoir	MPD	40 (%)