414D

5'-D(*GP*GP*GP*GP*CP*GP*CP*CP*CP*C)-3'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1996-12-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	45.504, 46.084, 28.465
Unit cell angles	90.00, 100.74, 90.00

Refinement procedure

Resolution	8.000 - 1.900
R-factor	0.174 *
RMSD bond length	0.005
RMSD bond angle	32.800 *
Data reduction software	MAR
Data scaling software	XDS
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.800
Rmerge	0.062
Total number of observations	41278 *
Number of reflections	10033
Completeness [%]	92.4
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5	291 *	pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K
1	Vapor diffusion, sitting drop *	6.5	291 *	pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K
1	Vapor diffusion, sitting drop *	6.5	291 *	pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K
1	Vapor diffusion, sitting drop *	6.5	291 *	pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SODIUM CACODYLATE
2	1	1	MGCL2
3	1	1	SPERMINE
4	1	1	MPD
5	1	2	MPD

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	DNA	2 (mM)
2	1	drop	sodium cacodylate	20 (mM)
3	1	drop		30 (mM)
4	1	drop	spermine tetrachloride	2 (mM)
5	1	drop		5 (%)
6	1	reservoir	MPD	50 (%)