411D
DUPLEX [5'-D(GCGTA+TACGC)]2 WITH INCORPORATED 2'-O-METHOXYETHYL RIBONUCLEOSIDE
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 297 |
Detector technology | IMAGE PLATE |
Collection date | 1996-01-03 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.930, 44.590, 45.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.930 |
R-factor | 0.168 |
Rwork | 0.168 |
R-free | 0.21000 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 22.700 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.070 |
Number of reflections | 4894 |
Completeness [%] | 92.7 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | pH 6.00, VAPOR DIFFUSION, HANGING DROP | |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | pH 6.00, VAPOR DIFFUSION, HANGING DROP | |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | pH 6.00, VAPOR DIFFUSION, HANGING DROP | |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | pH 6.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | SODIUM CACODYLATE | ||
2 | 1 | 1 | MAGNESIUM ACETATE | ||
3 | 1 | 1 | (NH4)2SO4 | ||
4 | 1 | 2 | SODIUM CACODYLATE | ||
5 | 1 | 2 | MAGNESIUM ACETATE | ||
6 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | oligonucleotide | 0.8 (mM) | |
2 | 1 | drop | sodium cacodylate | 25 (mM) | |
3 | 1 | drop | magnesium acetate | 7.5 (mM) | |
4 | 1 | drop | ammonium sulfate | 850 (mM) | |
5 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
6 | 1 | reservoir | magnesium acetate | 15 (mM) | |
7 | 1 | reservoir | ammonium sulfate | 1.7 (M) |