410D
DUPLEX [5'-D(GCGTA+TACGC)]2 WITH INCORPORATED 2'-O-ETHOXYMETHYLENE RIBONUCLEOSIDE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-14 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.620, 44.080, 46.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| R-free | 0.18400 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.210 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.089 |
| Number of reflections | 6524 |
| Completeness [%] | 92.7 |
| Redundancy | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | pH 6.00, VAPOR DIFFUSION, HANGING DROP | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | pH 6.00, VAPOR DIFFUSION, HANGING DROP | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | pH 6.00, VAPOR DIFFUSION, HANGING DROP | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | pH 6.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | BACL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | MPD | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | oligonucleotide | 1.2 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 3 | 1 | drop | sodium chloride | 40 (mM) | |
| 4 | 1 | drop | spermine tetrahydrochloride | 6 (mM) | |
| 5 | 1 | drop | barium chloride | 10 (mM) | |
| 6 | 1 | drop | 5 (%) | ||
| 7 | 1 | reservoir | MPD | 35 (%) |
