409D
CRYSTAL STRUCTURE OF AN RNA R(CCCIUGGG) WITH THREE INDEPENDENT DUPLEXES INCORPORATING TANDEM I.U WOBBLES
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10-02 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.440, 43.410, 49.390 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | R(CCCCGGGG) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 8.000 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.910 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.048 | 0.183 |
| Number of reflections | 4156 * | |
| <I/σ(I)> | 10.7 | 2.3 |
| Completeness [%] | 87.4 * | 61 * |
| Redundancy | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | pH 5.00, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CACODYLATE | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | SPERMINE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 1 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 3 | 1 | drop | magnesium chloride | 10 (mM) | |
| 4 | 1 | drop | spermine tetrachloride | 25 (mM) | |
| 5 | 1 | drop | 2.4 (%) | ||
| 6 | 1 | reservoir | MPD | 40 (%) |
