405D
STRUCTURE OF A 16-MER RNA DUPLEX R(GCAGACUUAAAUCUGC)2 WITH WOBBLE LIKE A.C MISMATCHES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-22 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | H 3 |
| Unit cell lengths | 42.560, 42.560, 124.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| R-factor | 0.17 |
| Rwork | 0.170 |
| R-free | 0.28900 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 6.900 * |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.350 |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.089 |
| Number of reflections | 2821 * |
| Completeness [%] | 86.4 * |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | KCL | ||
| 2 | 1 | 1 | CACL2 | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | PEG 400 | ||
| 5 | 1 | 1 | ETHYLENE GLYCOL | ||
| 6 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 0.1 (M) | ||
| 2 | 1 | drop | 0.01 (M) | ||
| 3 | 1 | drop | Na HEPES | 0.05 (M) | |
| 4 | 1 | drop | PEG400 | 10 (%) | |
| 5 | 1 | reservoir | PEG | 30 (%) | |
| 6 | 1 | reservoir | NaHEPES | 0.05 (M) |
