405D
STRUCTURE OF A 16-MER RNA DUPLEX R(GCAGACUUAAAUCUGC)2 WITH WOBBLE LIKE A.C MISMATCHES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04-22 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | H 3 |
Unit cell lengths | 42.560, 42.560, 124.050 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.500 |
R-factor | 0.17 |
Rwork | 0.170 |
R-free | 0.28900 |
RMSD bond length | 0.005 |
RMSD bond angle | 6.900 * |
Data reduction software | R-AXIS |
Data scaling software | R-AXIS |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.350 |
High resolution limit [Å] | 2.500 * |
Rmerge | 0.089 |
Number of reflections | 2821 * |
Completeness [%] | 86.4 * |
Redundancy | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | KCL | ||
2 | 1 | 1 | CACL2 | ||
3 | 1 | 1 | HEPES | ||
4 | 1 | 1 | PEG 400 | ||
5 | 1 | 1 | ETHYLENE GLYCOL | ||
6 | 1 | 2 | PEG 400 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | 0.1 (M) | ||
2 | 1 | drop | 0.01 (M) | ||
3 | 1 | drop | Na HEPES | 0.05 (M) | |
4 | 1 | drop | PEG400 | 10 (%) | |
5 | 1 | reservoir | PEG | 30 (%) | |
6 | 1 | reservoir | NaHEPES | 0.05 (M) |